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Biopolymers Facility at Harvard Medical School
The Affymetrix core at the Biopolymers Facility offers a full range of services for Gene
Chip processing. Please read the
submission details
page carefully before placing an order.
If you have any additional questions, please contact our Affymetrix core via email at
affymetrix@genome.med.harvard.edu.
Affymetrix services at the Biopolymers Facility include probe labeling,
chip hybridization, chip scanning and data analysis. All samples are run through a rigorous
quality control check before processing. Orders may be placed online for full or partial
service. Please include any special instructions in the comment field of our ordering form.
Full Service includes a six-step process to complete your
analysis.
The GeneChip Mapping 10K Array and Assay Set offer the ability to generate
over 10,000 genotypes on a single array using an innovative assay that
eliminates the need for locus-specific PCR. This assay requires only 250ng
of DNA for each sample, and provides more than 99.6% accuracy on a proven platform.
Our double amplification service is designed for low yield samples of
RNA derived from techniques such as cell sorting and laser capture. While normal sample
labeling requires 10ug (10,000ng) total RNA, double amplification allows us to start with
as little as 100ng total RNA. Concentrations must be no less than 10ng/ul.
All samples are processed using the most current Affymetrix equipment
and standardized protocols to assure high quality, consistent results. The BPF is equipped
with the following:
- One 2100 Bioanalyzer, Agilent Technologies
- One 8453 UV/Vis Spectrophotometer, Agilent Technologies
- Three GeneChip 400 Fluidics Stations, Affymetrix Inc.
- Two GeneChip 320 Hybridization Oven, Affymetrix Inc.
- One GeneArray Chip Scanners, HP/Affymetrix Inc.
To help our clients analyze the vast amount of data generated from
GeneChip analysis the Biopolymers Facility is equipped with a number of Bioinformatics
software tools. Access to our computer facility may be reserved in advance through our
online sign up sheet.
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Full service includes a six-step process to complete your analysis.
Expression Chip Processing does not include the cost of the GeneChip itself.
| Service |
Harvard/Partners |
Non-Harvard/Academic |
Commercial |
| Full Affy Service |
$400.00 |
$438.32 |
Note below |
| Double Amp. |
$500.00 |
$547.90 |
Note below |
| Frag+Hyb Service |
$200.00 |
$219.16 |
Note below |
| Hyb Service |
$150.00 |
$164.37 |
Note below |
| Scan Service |
$75.00 |
$82.18 |
Note below |
| Full 10K Mapping Serivce |
$325.00 |
$356.13 |
Note below |
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Commercial Institutions must contact the facility director for pricing.
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| Instructions |
| Step 1. |
Log into the BPF Website http://genome.med.harvard.edu and go to the "Place Order" section. Click on the "Place Order" link located within the main Affymetrix Ordering page. |
| Step 2. |
Enter the number of chips and type of services you would like to order, inlcuding any comments for our technicians. |
| Step 3. |
Enter sample names and corresponding concentrations for each of the chips requested. Please review your chip order to make sure the chip and service types are correct before proceding. |
| Step 4. |
Place each sample in a seperate RNase free microtube and label with the appropriate names given during the ordering process. Please make sure the labels are clearly marked as not to cause any delays during your chip processing. |
| Step 5. |
Drop your samples off directly at the Biopolymers Facility or mail them using the carrier of your choice. Aways ship or deliver RNA samples on dry ice, we do not take responsability for improperly packed customer samples. |
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Please direct questions or comments to our Affymetrix core at:
affymetrix@genome.med.harvard.edu.
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