Biopolymers Facility at Harvard Medical School

Oligonucleotide Synthesis

The Biopolymers Facility offers oligionucleotide synthesis at substantial discounts through third party vendors. All orders will be processed and handled at the BPF and then delivered to the client by a BPF staff member.

If you have any questions please contact our oligo processing unit via e-mail at oligos@genome.med.harvard.edu

Overview

The Biopolymers Facility has arranged discounted pricing for oligonucleotide synthesis through two third party vendors: Invitrogen (Life Technologies) and Integrated DNA Technologies (IDT). Our extensive client base and electronic ordering system allow us to group multiple requests for oligo synthesis together in one order, and pass the savings on to you.

Oligo orders can be placed through the BPF website at any time of day. During the week our staff gathers all of your synthesis requests and sends one order to the vendors at 2:00pm. All orders placed through our system after 2:00PM will be included in the next order request.

Direct shipping to the Biopolymers Facility allows for rapid turnaround time. Most oligonulceotides requests for the lowest scale and no additional purifications or modifications will be delivered to the client within two or three business days. Any further processing such as 5’ or 3’ modifications will require additional time to synthesize and deliver oligos to your lab.

Direct Delivery

Once oligos are received in bulk at the Biopolymers Facility our staff will sort and package the individual orders for distribution to our clients. In order to assure prompt delivery please maintain accurate location information on our web database. You can check and update your address information online under My Account – Edit Account. The BPF is not responsible for oligo orders once they have been delivered to the location specified in your account.

If you have any questions, please feel free to contact us at oligos@genome.med.harvard.edu or call our facility at 617-432-7481.

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Pricing

The price of each oligonucleotide ordered depends upon synthesis scale, purification type and length. Below is a chart detailing the various options available through our facility. If you wish to order something that you cannot find in the chart below, please email us at oligos@genome.med.harvard.edu to determine if we can obtain the specifications you desire.

Pricing for IDT
Scale 25 nMol 100 nMol 250 nMol 1 uMol
Yield > 2 OD > 6 OD > 15 OD > 40OD
Cost per base $0.24 $0.39 $0.64

$1.19

Purification Cost (Per Oligo)
Desalted $0.00 $0.00 $0.00 $0.00
Page n/a $40.00 $54.50 $95.00
Dual Page & HPLC n/a $81.00 $120.00 $185.00
RNase Free HPLC n/a $70.00 $85.00 $145.00
Modified Bases Cost (Per Oligo)
Mixed Based $0.00 $0.00 $0.00 $0.00
All Other Modifications Inquire Inquire Inquire Inquire

Pricing for IDT Plates
Scale 25 nMol 100 nMol 250 nMol 1 uMol
Cost per base $0.19 $0.29 $0.48

$0.95

Additional Costs
Dry Shipping Free
Wet Shipping $45.00 per order (6 plates max)
Plate of Remainders
(single oligo per well)
$96.00

Plate of Remainders
(dual oligos per well)

$192.00
Tubes of Remainders $240.00

Pricing for Invitrogen/Life Technologies
Scale 25 nMol 50 nMol 200 nMol 1 uMol
Yield > 2 OD > 20 OD > 20 OD > 50 OD
Cost per base $0.24 $0.34 $1.04

$3.05

Purification Cost (Per Oligo)
Desalted $0.00 $0.00 $0.00 $0.00
Cartridge T-On n/a $22.5 $25.50 $32.00
Cartridge T-Off n/a $22.5 $25.50 $32.00
HPLC n/a n/a $78.75 $105.00
Page n/a n/a $78.75 $135.50
Modified Bases Cost (Per Oligo)
Mixed Based $0.00 $0.00 $0.00 $0.00
All Other Modifications Inquire Inquire Inquire Inquire

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