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Biopolymers Facility at Harvard Medical School
The DNA Sequencing core at the Biopolymers Facility offers a
variety of services to fit your needs. Our basic sequencing service includes
BigDye labeling and CleanSeq purification, as well as 3730xL Analysis. The basic service is
available for both individual sample orders and whole plate orders.
Clients ordering in bulk through our whole plate option may also
order partial services, or include one of our pre-sequencing services: PCRClean or
MiniPrep. These two purification services include our basic service through analysis.
Please see our submission instructions for details on how to prepare your samples for
accurate and timely processing.
If you have any additional questions, please contact our sequencing
core via email at dnaseq@genome.med.harvard.edu.
The basic sequencing service at the Biopolymers Facility
begins with a cycle sequencing reaction using Dye Labeled Terminators
(BigDye 3.1) to generate extension products from client template DNA. The
reaction is then purified using a magnetic SPRI chemistry from Agencourt before
being processed on a 3730xL DNA Analyzer from Applied Biosystems. All sequencing data is
checked using our quality control software. We recommend viewing sequences with free software FinchTV.
Turn around time is between 24-48 hours for most samples.
The BPF requires samples to be submitted in a standard tube
that will fit our automated pipeline. These microtubes can be ordered
through the BPF using our Reagents and Supplies section.
We highly reccomend using the lables we have in stock for tube labeling.
Purified PCR products and plasmids may be submitted in either
15ul or 30ul volumes along with a primer in the same reaction tube. Any sample
submitted in a 30ul volume will be eligible for a FREE rerun. Rerun samples are
selected based on their score in our quality control software. All samples with
a PHRED score below 20 are automatically considered for a rerun unless additional
factors make it clear that the template may be the cause of error. All quality
scores are posted along with sample data.
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Our basic sequencing service includes dideoxynucleotide
terminator PCR and reaction cleanup, along with capillary electrophoresis
and signal analysis. Discounts are available for bulk orders and will be
billed at the discount price x 96. Bulk sample sets will be assigned one full
96-well PCR plate for reactions and analysis. There is no need to fill a
full plate, but please note that you will be billed for every available
position.
For our clients submitting in bulk we also offer partial
services. BigDye 3.1 labeled fragments may be submitted for CleanSeq and
Analysis. Purified samples ready for sequencing may be submitted for 3730xL
Analysis Only.
In addition to our basic sequencing service, the BPF also
offers purification services for whole plate orders of PCR products and
bacterial pellets. For details on how to prepare your samples please see our
submission instructions for whole plate orders.
Clients must supply sequencing primers
along with their unpurified samples.
| Service |
Harvard/Partners |
Non-Harvard Academic |
Non-Harvard Biotech |
| 96-Well (1 Plate) |
$4.50 |
$4.93 |
$5.40 |
| 96-Well (2 Plates) |
$4.00 |
$4.38 |
$4.80 |
| 96-Well (3 Plates) |
$3.50 |
$3.84 |
$4.20 |
| 96-Well (4 Plates or more) |
$2.00 |
$2.19 |
$2.40 |
| 384-Well (1 Plate or more) |
$2.00 |
$2.19 |
$2.40 |
| Standard Individual Sample (1 to 24) |
$8.50 |
$9.31 |
$10.20 |
| Standard Individual Sample (25 to 48) |
$7.50 |
$8.22 |
$9.00 |
| Standard Individual Sample (49 or more) |
$5.75 |
$6.30 |
$6.90 |
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| Service |
Harvard/Partners |
Non-Harvard Academic |
Non-Harvard Biotech |
| PCRClean, Standard Bulk Order 96-Well (1 Plate) |
$6.00 |
$6.57 |
$7.20 |
| PCRClean, Standard Bulk Order 96-Well (2 Plates) |
$5.50 |
$6.03 |
$6.60 |
| PCRClean, Standard Bulk Order 96-Well (3 Plates) |
$5.00 |
$5.48 |
$6.00 |
| PCRClean, Standard Bulk Order 96-Well (4 or more Plates) |
$3.50 |
$3.84 |
$4.20 |
| PCRClean, Standard Bulk Order 384-Well (1 Plate or more) |
$3.50 |
$3.84 |
$4.20 |
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| Service |
Harvard/Partners |
Non-Harvard Academic |
Non-Harvard Biotech |
| MiniPrep 96-Well Plate |
$1.50 |
$1.64 |
$1.80 |
| MiniPrep Tube |
$2.00 |
$2.19 |
$2.40 |
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| Service |
Harvard/Partners |
Non-Harvard Academic |
Non-Harvard Biotech |
| SEQClean & Instrument Run (1 to 3 96-Well Plates) |
$2.50 |
$2.74 |
$3.00 |
| SEQClean & Instrument Run (4 or more 96-Well Plates) |
$1.50 |
$1.64 |
$1.80 |
| SEQClean & Instrument Run (1 or more 384-Well Plates) |
$1.50 |
$1.64 |
$1.80 |
| 3730xL Instrument Run Only (1 to 3 96-Well Plates) |
$1.50 |
$1.64 |
$1.80 |
| 3730xL Instrument Run Only (4 or more 96-Well Plates) |
$1.00 |
$1.10 |
$1.20 |
| 3730xL Instrument Run Only (1 or more 384-Well Plates) |
$1.00 |
$1.10 |
$1.20 |
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** Non BPF tubes are automatically upcharged $1.00 per sample for labor/handling and the correct tube.
| Company |
Item Name |
Quantity |
Harvard/Partners |
Non-Harvard Academic |
Non-Harvard Biotech |
| USA Scientific BPF Sequencing Supplies |
8 Place Decapper |
each |
$5.95 |
$6.52 |
$7.14 |
| USA Scientific BPF Sequencing Supplies |
Bag of 960 Tubes |
each |
$29.50 |
$32.33 |
$35.40 |
| USA Scientific BPF Sequencing Supplies |
Box with insert |
each |
$1.40 |
$1.53 |
$1.68 |
| USA Scientific BPF Sequencing Supplies |
CapCluster - 8 Pack |
8 Pack |
$.35 |
$.38 |
$.42 |
| USA Scientific BPF Sequencing Supplies |
CapCluster - 96 Pack |
96 Pack |
$4.10 |
$4.49 |
$4.92 |
| USA Scientific BPF Sequencing Supplies |
CapMat |
each |
$4.60 |
$5.04 |
$5.52 |
| USA Scientific BPF Sequencing Supplies |
Custom 0.5 ml Tube - 8 Pack |
8 Pack |
$.25 |
$.27 |
$.30 |
| USA Scientific BPF Sequencing Supplies |
Custom 0.5 ml Tube - 96 Pack |
96 Pack |
$2.95 |
$3.23 |
$3.54 |
| USA Scientific BPF Sequencing Supplies |
Individual Caps - 8 Pack |
8 Pack |
$.35 |
$.38 |
$.42 |
| USA Scientific BPF Sequencing Supplies |
Individual Caps - 96 Pack |
96 Pack |
$3.85 |
$4.22 |
$4.62 |
| USA Scientific BPF Sequencing Supplies |
Lo-Bo Rack |
each |
$15.10 |
$16.55 |
$18.12 |
| USA Scientific BPF Sequencing Supplies |
Sheet of tags |
each |
$2.30 |
$2.52 |
$2.76 |
| USA Scientific BPF Sequencing Supplies |
Single Place Decapper |
each |
$5.00 |
$5.48 |
$6.00 |
| USA Scientific BPF Sequencing Supplies |
Starter Kit |
each |
$9.95 |
$10.90 |
$11.94 |
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Due to customer demand, we are begining a trade in program for the new tubes. A lab may bring down Marsh 1.2 mL tubes and exchange them for the new 0.5 mL tubes. Please contact dnaseq@genome.med.harvard.edu to arrange your tube exchange.
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All samples for our basic sequencing service must be submitted in 0.5 ml microtubes.
The minimum volume is 15ul including template and primer. For concentration
information and step-by-step submission instructions please follow the links
above for your preferred submission size.
You may also submit samples for basic sequencing in 30ul
volumes, which will automatically qualify your samples for free reruns in the
event of a failed reaction. Again please see the details above for concentration
information.
Samples submitted for Whole Plate Orders receive our discounted
ordering price and are eligible for pre-sequencing or partial service options. Follow
the link above for whole plate instructions regarding purification of PCR products
and plasmids as well as BigDye cleanup and 3730xL Analysis. Partial plates are
acceptable as whole plate orders but will be billed as a full unit.
The Biopolymers Facility requires all users to provide their own
primer for each DNA sequencing sample. If you need a standard primer such as T7 you
can order it through our oligonucleotide synthesis service and the primer will be available here for pick up. For primer design hints and primer sequences see follow our
information link above.
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