| Instructions |
| 1. |
On the web at http://genome.med.harvard.edu
login with a valid ID and password. Navigate to the sequencing section and then click
Order Individual Template. Type the number of templates you wish to submit and select
either 15ul or 30ul total volume. Fill in your template name, and primer and select
your expected read length. Confirm and complete your order, then print the requisition
sheet.
|
| 2. |
In the required microtube prepare your samples with both template and
primer according to the chart below:
| |
|
30 ul |
| Template |
PCR product |
12.5 ng/100 base pairs |
25 ng/100 base pairs |
| Double Strand DNA (plasmid) |
500 ng |
1000 ng |
| Single Stand DNA |
100 ng |
200ng |
| Note: contact us for details on BAC sequencing. |
| Primer |
|
4 pmol |
8 pmol |
|
| 3. |
Line up samples tubes in the order in which they are listed on
your requisition sheet. Seal tubes with caps. |
| 4. |
Please label each tube only with the unique sample number listed
next to the corresponding sample name on your requisition sheet.
Each sample should have a unique number that follows the order
your have arranged your tubes. Sample names should not be
written on tubes.
We highly recommend using the labels we have in stock for tube labeling.
|
| 5. |
See our list of Drop off locations. |
| You can track the status of your samples online under
View My Orders and when your order is complete
download the data in AB1 format. |
