Bioanalyzer & TapeStation

The BPF is equipped with an Agilent Bioanalyzer and an Agilent 2200 TapeStation. Both instruments can analyze DNA and RNA. These multipurpose platforms are designed as microfluidic alternatives to gel electrophoresis. The "lab-on-a-chip" technology integrates sample separation with automated analysis to provide reproducible data faster and more economically than traditional methods. Results can be viewed as an electropherogram or as a standard gel-like image. For more details about the Agilent systems please see their brochures.

The Bioanalyzer and TapeStation have the ability to determine the quantity and quality of Total RNA, mRNA, cDNA, cRNA, ssDNA as well as dsDNA. These technologies can also be used to evaluate Next-Gen libraries. There are a variety of LabChips and ScreenTapes to choose from depending on the type and size range of your samples. The Boanalyzer utilizes a 25bp marker to standardize each lane to the ladder that is run with each chip type. The marker and the ladder aid in determining the size and quality of the sample. The TapeStation includes a ladder to be run with each ScreenTape to perform similar analyses. You will receive a PDF file as well as an instrument software specific file (.D1K, .D5K, .R6K, etc for the TapeStation, .xad for the Bioanalyzer). You must download Agilent software to view and manipulate the instrument specific files. Links to the downloads are available in our Data Analysis section.

  • Agilent Bioanalyzer
  • Agilent 2200 TapeStation

Please bring your order form and samples to the Biopolymers Facility located at NRB Room 0088 (9:00 am - 5:00 pm, Monday - Friday).

All samples should meet the following criteria:

  • Provide at least 2uL of each sample.
  • Samples must meet concentration criteria, listed below. Please dilute your samples if they are out of range.
  • Submit samples in a 0.2mL PCR tube.
  • Label tubes with sample name.
  • Samples should be on dry ice.
Services
Instrument Chip or ScreenTape Nucleic Acid Type Concentration Range
Agilent 2200 TapeStation D1000 DNA 0.1 ng/ul to 50 ng/ul
Agilent 2200 TapeStation Genomic DNA 10 ng/ul to 100 ng/ul
Agilent 2200 TapeStation High Sensitivity D1000 DNA 10 pg/ul to 1000 pg/ul
Agilent 2200 TapeStation High Sensitivity D5000 DNA 10 pg/ul to 1000 pg/ul
Agilent 2200 TapeStation High Sensitivity RNA RNA 1000 pg/ul to 25000 pg/ul
Agilent 2200 TapeStation RNA RNA 25 ng/ul to 500 ng/ul
Agilent BioAnalyzer DNA High Sensitivity DNA 5 pg/ul to 500 pg/ul
Agilent BioAnalyzer RNA Nano Total RNA 5 ng/ul to 500 ng/ul
Agilent BioAnalyzer RNA 6000 Nano mRNA 25 ng/ul to 250 ng/ul
Agilent BioAnalyzer RNA Pico Total RNA 50 pg/ul to 5000 pg/ul
Agilent BioAnalyzer RNA 6000 Pico mRNA 250 pg/ul to 5000 pg/ul
Agilent BioAnalyzer Small RNA small RNA* 50 pg/ul to 2000 pg/ul
* Size Range for small RNA is 6 to 150 nucleotides.
Harvard Internal
Harvard Affiliate/Academic
Commercial
Service Setup Fee Per Sample Setup Fee Per Sample Setup Fee Per Sample Samples per Setup Fee
Agilent 2200 TapeStation D1000 $12.67 $5.28 $14.57 $6.07 $16.47 $6.86 15
Agilent 2200 TapeStation Genomic DNA $13.80 $6.57 $15.87 $7.55 $17.94 $8.54 15
Agilent 2200 TapeStation High Sensitivity D1000 $14.62 $6.44 $16.81 $7.41 $19.00 $8.37 15
Agilent 2200 TapeStation High Sensitivity D5000 $14.62 $7.15 $16.81 $8.23 $19.00 $9.30 15
Agilent 2200 TapeStation High Sensitivity RNA $14.44 $5.40 $16.61 $6.21 $18.77 $7.02 15
Agilent 2200 TapeStation RNA $13.67 $5.05 $15.72 $5.81 $17.77 $6.56 15
Agilent BioAnalyzer - DNA High Sensitivity $114.43 $3.27 $131.60 $3.76 $148.76 $4.25 11
Agilent BioAnalyzer - RNA Nano $88.31 $3.27 $101.55 $3.76 $114.80 $4.25 12
Agilent BioAnalyzer - RNA Pico $91.43 $3.27 $105.15 $3.76 $118.86 $4.25 11
Agilent BioAnalyzer - Small RNA $104.46 $3.27 $120.13 $3.76 $135.80 $4.25 11

For additional protocols and documentation, please visit the Agilent website http://www.agilent.com/.

We review the traces from each LabChip or ScreenTape to look for run anomalies and will repeat samples that have poor data as a result of an instrument or reagent problem. Otherwise, it is your responsibility to evaluate the data for quality and concentration. For RNA a RNA Integrity Number (RIN) value (Bioanalyzer) or RNA Integrity Number Equivalent (RINe) value (TapeStation)is generated to represent RNA quality. More details regarding these values can be found on the Agilent website. You must download Agilent software to view and manipulate the instrument specific files