Library construction is the process of taking DNA or RNA from its "native" state to a form that can be run on the Illumina sequencer. The majority of protocols require fragmentation of the nucleic acid material to a specific size range. The BPF offers enzymatic fragmentation, as well as shearing services using the Covaris M220 instrument. There are dozens of methods and kits for the production of Illumina compatible libraries available as well as multiple automation platforms to run these methods. The BPF has adopted the Apollo Wafergen 324 and the Sciclone NGS G3 Workstation. We offer a variety of library methods on this instrument. Please see the Library Prep Options section below for a complete list of methods currently available from our core. If you are interested in a library prep service and do not see it below, please contact us. We are always happy to discuss options not yet listed!
- Apollo Wafergen 324 Robot
- Covaris M220
- MJ Research Tetrad DNA Engine Thermal Cycler
- Qiagen Qiagility Robot
- Perkin Elmer Sciclone NGS G3 Workstation
Please provide starting material as specified in the table below. If you are not able to determine the quantity or quality of your starting material, you should order Bioanalyzer or TapeStation services prior to ordering your library prep service.
Every library we produce is run on an Agilent 2200 TapeStation D1000 HS ScreenTape to assess concentration and size distribution of the library molecules. In addition, a QPCR assay is run to determine functional concentration of the final library in an effort to obtain optimal cluster densities prior to the sequencing process.
Quality Control services have a small additional fee. Further information regarding this service can be found in out NextGen Sequencing section.